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KMID : 0365819690090020105
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Journal of Pusan Medical College
1969 Volume.9 No. 2 p.105 ~ p.115
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Experimental Studies on Buffer Capacity of Cardiac Muscle and Electrocardiogram of :hypercapnic Rabbits
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Abstract
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The intracellular pH was determined from the cardiac muscles of 15 normal, 17 hypercapnic and 8 hyperkalemic rabbits, using 5, 5-dimethyl-2, 4-oxazolidinedione (DMO) method.
The relationship between intracellular and extracellular hydrogen ion concentrations and between intracellular gnd extracellular buffer capacities were observed.
Electrocardiograhic changes were studied on the hypercapnic rabbits which were produced by administration of 10 to 2070 carbon dioxide-oxygen mixtures and on the hyperkalemic rabbits which were produced by intravenous infusion of 1(C1 solution.
The results were obtained as follows;
1) Normal acid-base parameters of blood were pH 7.46, H+ concentration 35 nM/L, Pco2 30 mmHg, HC03- concentration 20. 4 mEq/L and those of cardiac muscles were pH 7. 04, H+ concentration 91 nM/L, HCO3; concentration 9.2 mEq/L.
2) Buffer curve in acute hypercapnic rabbits had a slope of 31. 2 mEq/L, pH and that of cardiac muscle 17.6 mEq/L, pH.
3) H+ concentration ratio between blood and cardiac muscle was 1: 2. 60 in normal rabbits, whereas it was 1:2.76 in hypercapnic rabbits. The relationship between H+ concentration of blood and cardiac muscle was expressed in an equation of(H+)i=-19.5+3. 05(H+)e.
4) Acute hyperkalemia induced acidosis in cardiac muscle as well as in blood.
5) It seemed that the electrocardiographic changes in acute hypercapnic rabbits were mainly correlated with the increase in plasma concentration of potassium rather than hydrogen ion.
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